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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
D4-4-Fluorobenzyl Chloride
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:1219804-10-0;unlabelled:352-11-4
- 规格:
1mg///5mg///10mg
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文献和实验Site-directed hydroxy radical mapping of nucleosome positions in vitro
). Alternatively, fragments can be isolated by restriction digestion of plasmids after large scale plasmid preparations (see note 9); DNA fragments should be strand-specifically labelled, for example with a fluorophore dye or radioisotope (see note 10
Polymerase Chain Reaction1.Add the following to a microfuge tube:10 ul reaction buffer1 ul 15 uM forward primer1 ul 15 uM reverse primer1 ul template DNA5 ul 2 mM dNTP8 ul 25 mM MgCl2or MgSO4(volume variable)water (to make up to 100 ul)2.Place tube
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
x dNTP mix: Digoxigenin-11-dUTP pre-mixed with other nucleotides (1 mmol/l dATP, 1 mmol/l dCTP, 1 mmol/l dGTP, 0.65 mmol/l dTTP, 0.35 mmol/l DIG-dUTP). (Boehringer Cat# 1277 065.) - 10x PBS: 80g NaCl, 2g KCl, 6.1g anhydrous Na2HPO4, 2g KH2PO4, H2
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