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- 文献和实验
- 技术资料
- 英文名:
Polyethylene Glycol Monostearate(n=approx. 45) (palmitate and stearate mixture),聚乙二醇单硬脂酸酯(n=约45) (棕榈酸酯和硬脂酸酯的混合物)
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
N/A
- 规格:
100mg///1g
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文献和实验Isolation of Microtubules (Bovine Brain)
)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis( -aminoethyl Ether) N,N,N',N'-tetraacetic acid) 0.5 mM MgCl Adjust pH to 6.4 8 M Glycerol in MT buffer Virtis homogenizer
Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
(all till the last drop!!!)7、gently break the pellet by tapping of the bottom of the tube.8、place the tube on 37℃ water bath; add slowly, dop by drop 1 ml of pre-warmed (37℃) 50% PEG 1500 (polyethylene glycol, Roche, Germany) continously stirring the cells
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