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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Polyethylene Glycol Monostearate(n=approx. 45) (palmitate and stearate mixture),聚乙二醇单硬脂酸酯(n=约45) (棕榈酸酯和硬脂酸酯的混合物)
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
N/A
- 规格:
100mg///1g
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文献和实验Isolation of Microtubules (Bovine Brain)
LEVEL II Materials Freshly removed bovine brain 2 Wire sieve (tea strainer) Microtubule buffer (MT buffer) 0.1 M MES (2-(N-Morphilino)ethanesulfonic acid) 1 mM EGTA (Ethylene Glycol-bis
(all till the last drop!!!)7、gently break the pellet by tapping of the bottom of the tube.8、place the tube on 37℃ water bath; add slowly, dop by drop 1 ml of pre-warmed (37℃) 50% PEG 1500 (polyethylene glycol, Roche, Germany) continously stirring the cells
Fusion is an important procedure to produce monoclonal antibodies (MoAb).
glycol, Roche, Germany) continously stirring the cells with the pipette tip (this procedure lasts for a period over 1 min) add 1 ml of pre-warmed to 37ѓC IMDM continously stirring the mixture (this procedure lasts for a period over 1 min).
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