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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Polyethylene glycol diacrylate, M.W. 8,000,聚乙二醇二丙烯酸酯, M.W. 8,000
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
N/A
- 规格:
100mg///1g
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文献和实验Crystallization of Kinesin Family Motor Proteins
(2-carboxyethyl)-phosphineReservoir: 20% polyethylene glycol 3350 in 0.2 M di-sodium hydrogen phosphate, pH 9.1Vinogradova et al., 2004KinISitting drop4°C10 - 20 mg/ml protein Reservoir: 1.4-1.8 M ammonium sulfate, 100 mM sodium acetate (pH 5.0), 200 mM sodium
should be between 8.4 - 8.9. Note : Lithium Acetate can be sterilized in the autoclave for 20 mins liquid cycle. Polyethylene glycol (PEG 50% w/v) The polyethylene glycol (PEG), MW 3350 (Sigma P3640) is made up to 50% (w/v) with dd water and filter
Cesium Chloride Purification of T7
1 molar. Centrifuge the lysate at 10,000 rpm for 10 min, Discard the cellular debris, and add 10 grams polyethylene glycol (PEG) m.w. 8000 (10% w/v) to the supernatant. Gently stir the mixture until the PEG has totally dissolved. Keep lysate on ice
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