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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
6-O-Sulphated Lewisa-BSA (3 atom spacer)
- 库存:
999
- 供应商:
智溯科ZSK
- 规格:
5mg///10mg
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文献和实验Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Immunohistochemistry on fixed, paraffin-embedded sections
7.5, to which 0.01% Tween 20 has been added (TBS-T). 2- Briefly blot the slides without letting them dry and then apply 3% human or pig serum in PBS-BSA-NaN 3 as a blocking agent (health hazard!). 3- incubate with the blocking for 10 min
DETECTION OF RECOMBINANT PROTEINS BY ELISA
of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie
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