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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
(Z)-isopropyl3-(3-(3,5-bis(trifluoromethyl)phenyl)-1H-1,2,4-triazol-1-yl)acrylate
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
1333152-22-9
- 规格:
10mg
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文献和实验RNA purification using TRizol--TRIZOL法提取RNA
Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopropyl alcohol (2-Propanol) (Fisher)75% Ethanol
Sauer:RNA Purification from E. coli
too low in RNA prep''d by resuspending E. coli in tri-reagent (called Trizol from here on) as the "lysis" step. Even though Trizol has phenol and guanidinium thiocyanate, and the samples were heated to 65 degrees for 30 minutes, the large RNAs just didn''t
Bisulfite sequencing of small DNA/cell samples
A: Preparation of genomic DNA from samples with more than 100 cells per sample Mix the collected mammalian cells (>100) with 50 µl of solution A (25 mM EDTA, 75 mM NaCl), 50 µl of solution B (10 mM EDTA, 10 mM Tri-HCl, pH 8.0, 1% SDS), add 2 µl
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