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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
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- 英文名:
Corning® BioCoat™ Poly-D-Lysine 25cm² Rectangular Canted Neck Cell Culture Flask with Vented Cap
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
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- 规格:
10
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文献和实验Establishment of a Primary Culture
in MEM at 1000 rpm for 10 minutes in a standard clinical centrifuge. Remove the supernatant and resuspend the pellet in 25 ml of fresh MEM + 10% FCS. Remove 0.1 ml of the culture and determine cell concentration and viability
. No. D8537) using a volume equivalent to half the volume of culture medium. Repeat this wash step if the cells are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area
of an ocular grid inserted into an inverted phase contrast microscope. Cell density (cells/cm2) is then plotted on a log scale against time in culture.Exercise 12.1 - Aseptic Cell Transfers Level I Figure 12.3 Flaming a wire loop and removing cap
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