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Corning® BioCoat™ Collagen I 150cm² Rectangular Canted Neck Cell Culture Flask with Vented Cap, 5/Pack, 5/Case
- 库存:
99
- 供应商:
北京孚博生物
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- 规格:
5
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文献和实验Endothelial Cell Systems - Instructions For Use
, strain number, cell type and date. Carefully open the medium bottle and transfer growth medium to new culture vessels to by adding 1 ml growth medium for every 5 cm2 surface area of the flask (1ml/5cm2). Example: 15 ml growth medium for a 75 cm2 flask
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
实验试剂 Buffer: Phosphate buffered saline with 0.1% bovine serum albumin and 2 mM EDTA, pH 7.4 (PBS w/0.1% BSA). Magnet (DynaMag™): See www.invitrogen. com/magnets for magnet recommendations. Culture medium: Advanced
100x Pen-Strep (P4333 or P0781) • 5 ml 100x sodium pyruvate (S8636 or P4562) • 5 ml beta-mercaptoethanol Stock solution = 3.5 µl diluted to 5 ml neat beta-maerpactoethanol (M7522) with tissue culture grade water (W3500) — make
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