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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷冻-20℃
- 英文名:
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[folate(polyethylene glycol)-5000] ammonium salt
- 库存:
999+
- 供应商:
上海维亦特生物科技有限公司
- CAS号:
1236288-25-7
别名:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[folate(polyethylene glycol)-5000] ammonium salt
吸湿性:Yes
分子式:C287H558N11O127P (average MW due to polydispersity of PEG)
分子量:6226.545
稳定性:1 Years
存储:冷冻-20°C
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文献和实验Materials YPD plates YPD 1 M sorbitol 182 g/l (Sigma S7547) 2 M sorbitol 36.4 g/100 ml SCE (per liter) 1 M sorbitol (182 g) 100 mM citric acid trisodium salt dihydrate (29.4 g)
OUTLINE Ammonium sulphate precipitation is used to purify a protein (in this case an immunoglobulin) from a big volume of liquid phase. PROTOCOL Slowly (!!!) add the solution of ammonium sulphate (40-50% final, v/v
xylene cyanole FF (Kodak T-1579) ddH2 O to 10 ml (store at -20deg.C). Alkaline lysis solution (NaOH/SDS) : 0.2 N NaOH, 1% SDS in ddwater. 20 ml of 1 N NaOH (or 0.8 gms) 10 ml of 10% SDS (or 1.0 gms) ddH2 O to 100 ml (make fresh) 15% Ammonium
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