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- 技术资料
- 保存条件：
冷冻-20℃
- 英文名：
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] ammonium salt
- 库存：
999+
- 供应商：
上海维亦特生物科技有限公司
- CAS号：
474922-22-0
别名：1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] ammonium salt
吸湿性：Yes
分子式：C139H271N4O57P (average MW due to polydispersity of PEG)
分子量：2941.642
稳定性：1 Year
存储：冷冻-20°C
溶解性：Solubility in Different SolventsSoluble in ethanol, DMSO, and Chloroform:Methanol (85:15) at 5mg/mL
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文献和实验Crystallization of Kinesin Family Motor Proteins
temperature11 mg/ml protein 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM MgCl2, 0.2 mM NaN3, 2 mM ADP combined 1:1 with 22% methyl ether PEG 2000, 100 mM NaCl, 2% ethylene glycol, 50 mM HEPES pH 7.0Gulick et al., 1998Ncd 281-700Sitting drop 4°C20 mg/ml
Site-directed hydroxy radical mapping of nucleosome positions in vitro
are 0.1-0.4µM nucleosome, 75mM Tris-Cl pH 7.5, 2µM ammonium ferrous sulphate, 4.8mM ascorbate, 0.05% hydrogen peroxide and monovalent salt as required. We find ascorbate to be a major limiting factor in the reaction. However, adding too much will affect the pH
Materials YPD plates YPD 1 M sorbitol 182 g/l (Sigma S7547) 2 M sorbitol 36.4 g/100 ml SCE (per liter) 1 M sorbitol (182 g) 100 mM citric acid trisodium salt dihydrate (29.4 g)
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