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- 文献和实验
- 技术资料
- 保存条件:
冷冻-20℃
- 英文名:
1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[azido(polyethylene glycol)-2000 ammonium salt
- 库存:
999+
- 供应商:
上海维亦特生物科技有限公司
- CAS号:
2315262-00-9
别名:1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[azido(polyethylene glycol)-2000 ammonium salt
分子式:C128H256N5O54P (average MW due to polydispersity of PEG)
分子量:2760.411
稳定性:1 Year
存储:冷冻-20°C
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文献和实验Crystallization of Kinesin Family Motor Proteins
temperature11 mg/ml protein 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM MgCl2, 0.2 mM NaN3, 2 mM ADP combined 1:1 with 22% methyl ether PEG 2000, 100 mM NaCl, 2% ethylene glycol, 50 mM HEPES pH 7.0Gulick et al., 1998Ncd 281-700Sitting drop 4°C20 mg/ml
常用化学试剂分子量 Molecular Weights of Commonly Used Chemicals
Ammonium Sulfate 132.14 Polyvinylpyrrolidone 40,000 Ampicillin 371.4 Potassium Acetate 98.14 ATP, disodium salt 605.19
Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
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