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- Omega Bio-Tek
- TQ2100-01
- 2025年12月16日
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- 技术资料
Taq DNA Polymerase is purified from E. coli. expressing a cloned
Thermus aquaticus DNA polymerase gene. This enzyme has both
a 5' → 3' DNA polymerase and a 5' → 3' exonuclease activity but
lacks a 3' → 5' exonuclease activity. The enzyme consists of a
single polypeptide with a molecular weight of approximately 94
kDa. Taq DNA polymerase is heat-stable and will synthesize DNA
at elevated temperatures from single-stranded templates in the
presence of a primer.
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文献和实验out the E.coli strain carrying the Taq polymerase gene on an LB amp plate and innoculate a 5 ml overnight culture with a single colony.• Innoculate a 1 liter culture of LB-amp with the 5 ml of overnight culture and grow to an A600 of 0.2.Add IPTG to a final
High Specificity PCR Amplification Using AccuPrime Taq DNA Polymerase
Taq DNA Polymerase 0.25 µl 0.5 µl 1 µl Autoclaved distilled water To 10 µl To 25 µl
Dideoxy Sequencing Reactions Using Taq Polymerase
Tuq DNA polymerase is a highly stable polymerase isolated from the thermophilic organism Thermus aquaticus . Because of its unique properties this enzyme has been extensively used for amplification of DNA fragments by the polymerase chain
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