- ￥380
- Omega Bio-Tek
- TQ2401-01
- 2025年12月15日
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- 文献和实验
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Description
The Omega Bio-tek’s M-MLV Revers Transcriptase is Moloney
Murine Leukemia Virus Revers Transcriptase, it is an
RNA-dependent DNA polymerase that can be in cDNA
synthesis with long messenger RNA templates. The M-MLV
RTase that has been engineered to reduce RNase H activity
and provide increased themal stability. The enzyme is used
to synthesize cDNA at a temperature range of 37-42°C,
providing increased specificity, higher yields of cDNA, and
more full-length product than other reverse transcriptase. 
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文献和实验50µl M-MLV Reverse Transcriptase (b) (200U/µl) 4000U 10000U RNase-free ddH2 O 1ml
3. a) Add RNA to beads. b) Heat to 70 C for 2 min and cool slowly to RT for 10 min. c) Bind beads. d) Remove liquid. 4. Resuspend beads in : 2.5 ul Buffer A* (200 mM Tris-HCl, pH 8.3,1.0 M KCl) 2.5 ul Buffer B* (30 mM MgCl2 and 15 mM
in : 2.5 ul Buffer A* (200 mM Tris -HCl, pH 8.3,1.0 M KCl) 2.5 ul Buffer B* (30 mM MgCl2 and 15 mM MnSO4) 20.0 ul dNTPs (2.5 mM each) 1.0 ul 32P-dCTP (5 uCi) 1.0 ul RNasin-Pharmacia 2.0 ul
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