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文献和实验实验试剂 1. Sterile deionized water (or TE buffer) 2. Absolute (or 95%) ethanol 实验设备 1. Protective eye-ware 实验步骤 1. MicroElute™ Gel Extraction Spin Protocol
Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
for an hour. The dried pellet is resuspended in TE buffer (1M Tris-HCl; 0.5M EDTA; pH 8.0) and left overnight on a rotator. DNA concentration is determined either by agarose gel electrophoresis or spectrophotometry
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