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- Omega Bio-Tek
- M1259-01
- 2026年03月18日
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文献和实验Mag-Bind® Plasmid Mega Magnetic Protocol
. The culture density should reach 3-4 x 109 per mL. It is strongly recommended that an endA negative strain of E.coli be used for routine plasmid isolation. Examples of such strains include DH5α® and JM109® . 2. Harvest the bacterial cells
with ethanol into the tube. Resuspend Mag-Bind particles pellet by vortexing. 15. Place the tube onto a magnetic separation device to magnetize the MagSi particles. Carefully remove and discard the cleared supernatant. 16. Wash MagSi particles
Mag-Bind RX Plasmid Isolation Protocol
at room temperature. 3. Remove the tape and discard supernatant into a waste container. Dry the plate by placing upside-down on a paper towel to remove excess media. 4. Add 90 ul Solution I/RNase A and 30ul Mag-Bind Particle LC to the bacterial
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