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文献和实验E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
ethanol (room temperature, 96-100%). Gently mix by inverting tube 6-7 times and incubate at room temperature for1-2 minutes. Plasmid DNA Purification with the HiBind® DNA Mini Column II: 8. Equilibrate the HiBind® DNA column: Add 100ul
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
实验步骤 1. Inoculate 5ml LB/ampicillin (50 ug/ml) medium placed in a 10-20 ml culture tube with E.coli carrying desired plasmid and grow at 37°C with agitation for 12-16 h. It is strongly recommended that an endA negative
Plasmid Mini Purification Protocol
Important Notes Before Starting • New users are strongly recommended to read the QIAGEN handbook before starting the procedure. •Before using the kit for the first time, dissolve the lyophilized RNA se A provided Buffer PI . Buffer PI
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