Endo-Free Plasmid Maxi Kit-fas

E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol

with occasional mixing. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. (Store Solution II tightly capped when not in use.) 4. Add 250 ul ice-cold Buffer N3 and mix gently but throughly by inverting tube several times

Fastfilter Plasmid Maxi Kit Spin Protocol

with downstream applications. Elution Plasmid DNA From HiBindTM DNA Maxi column: Optional: For maximal yield and high concentration of plasmid, see alternative protocol of elution on page 7. For fast elution, proceed step 15-16. 15

E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol

实验步骤   1. Inoculate 5ml LB/ampicillin (50 ug/ml) medium placed in a 10-20 ml culture tube with E.coli carrying desired plasmid and grow at 37°C with agitation for 12-16 h. It is strongly recommended that an endA negative