- ￥495
- 博日
- BSB35L1B1
- 国产
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海艾研生物科技有限公司
- 规格：
500T
| BioEasy Master Mix( Probe) II | BSB35L1B | 500T | 盒 | 900 | 495 | 400 | 320 | 300 | / | 50，100盒 |
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文献和实验TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
Preparation and purification of biotin-labeled probe for ISH
, however the size of the probe is relatively easier to control by nick translation. II. Purification of the biotin-labeled probe. Start 20 min before the end of the nick translation incubation. Plug the bottom of a 1 ml tuberculin syringe
cDNA synthesis using superscript II
SuperScript II 1 µl water 4 µl Prepare this mix as a master mix and add then 20 µl to each reaction. Synthesize the cDNA in a thermocycler: 1.
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