- ￥1338 - 11148
- abinScience
- France
- VK589313
- 2025年07月14日
- ELISA
- Human
- Dengue virus type 4 (strain Dominica/814669/1981) (DENV-4)
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1, kappa
- 形态：
Liquid
- 保存条件：
-20°C
- 克隆性：
单克隆
- 适应物种：
Dengue virus type 4 (strain Dominica/814669/1981) (DENV-4)
- 保质期：
12 months
- 级别：
一抗
- 供应商：
武汉益普生物科技有限公司
- 宿主：
Human
- 应用范围：
ELISA
- 浓度：
1 mg/ml
- 抗体英文名：
Anti-DENV-4 Envelope protein E/EDI domain Antibody (5H2)
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1338.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥2288.0 |
| 规格： | 1mg | 产品价格： | ￥11148.0 |
| Product name | Anti-DENV-4 Envelope protein E/EDI domain Antibody (5H2) |
| Catalog No. | VK589313 |
| Target | Genome polyprotein, Envelope protein E |
| Clonality | Monoclonal |
| Host species | Human |
| Species reactivity | Dengue virus type 4 (strain Dominica/814669/1981) (DENV-4) |
| Applications | ELISA |
| Isotype | IgG1, kappa |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
To immunostain using enzyme-coupled secondary antibodies: i. Rinse slides in PBS and remove excess solution. ii. Add 40 µL of secondary antibody (e.g., anti-rabbit IgG [Fc] HRP conjugate, 1:100 dilution
To fix, 1/10 volume of 37 formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended then recovered
step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
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