- ￥1176 - 9828
- abinScience
- France
- HV462043
- 2025年07月14日
- IHC, WB
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a, kappa
- 形态：
Liquid
- 保存条件：
-20°C
- 克隆性：
单克隆
- 保质期：
12 months
- 级别：
一抗
- 供应商：
武汉益普生物科技有限公司
- 宿主：
Mouse
- 应用范围：
IHC, WB
- 浓度：
1 mg/ml
- 抗体英文名：
Anti-FGF13/FHF2 Antibody (N225A/10)
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1176.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1956.0 |
| 规格： | 1mg | 产品价格： | ￥9828.0 |
| Product name | Anti-FGF13/FHF2 Antibody (N225A/10) |
| Catalog No. | HV462043 |
| Target | FGF13, FHF-2, Fibroblast growth factor homologous factor 2, FGF-13, Fibroblast growth factor 13, FHF2 |
| Clonality | Monoclonal |
| Host species | Mouse |
| Applications | IHC, WB |
| Isotype | IgG2a, kappa |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
, add ddH2 O up to 1 L Gluta x50, 1 L, pH 6.0 = glutamine, 10.8 g (1.48 mM) + HCl, 0.5N, 100 ml -> heat to 37ѓC, add ddH2 O up to 1 L aminopterine x100, 0.22-0.45 µm-filtered, store at -20ѓC = aminopterine, 4.4 mg (3.8µM)+ ddH2 O
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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