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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1, kappa
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
单克隆
- 适应物种:
Dengue virus type 2 (strain Thailand/PUO-218/1980) (DENV-2),Dengue virus type 2 (strain Jamaica/1409/1983) (DENV-2)
- 保质期:
12 months
- 级别:
一抗
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Human
- 应用范围:
ELISA, Neutralization
- 浓度:
1 mg/ml
- 抗体英文名:
InVivoMAb Anti-DENV2 Protein prM/prM Antibody (2H2)
- 规格:
100μg/1mg
| 规格: | 100μg | 产品价格: | ¥2588.0 |
|---|---|---|---|
| 规格: | 1mg | 产品价格: | ¥9188.0 |

| Product name | InVivoMAb Anti-DENV2 Protein prM/prM Antibody (2H2) |
| Catalog No. | VK494030 |
| Target | Genome polyprotein, Protein prM, Peptide pr, Small envelope protein M, Matrix protein, Envelope protein E, Non-structural protein 1, NS1 |
| Clonality | Monoclonal |
| Host species | Human |
| Species reactivity | Dengue virus type 2 (strain Thailand/PUO-218/1980) (DENV-2),Dengue virus type 2 (strain Jamaica/1409/1983) (DENV-2) |
| Applications | ELISA, Neutralization |
| Isotype | IgG1, kappa |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. Not suitable for clinical or therapeutic use. |

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文献和实验Staining for Surface Receptors
2, 16g NaCl, 6g Tris base, 0.4g KCl, 0.294g CaCl2 . 2H2O; pH 7.4 with conc. HCl. Blotto -- Total Volume 10mL, 0.3g dry milk (3%), 100ul 10% Triton X-100 (0.1%), 100ul 100mM CaCl2 (1mM), 500ul 1M Tris-Cl pH 7.5 (50mM ). Fixative
)1:1000. 9. Wash 3x. 10. Second Blotto block as above, 5'. 11. Aspirate Blotto, and apply secondary Ab. Incubate 20' in the dark. Dilute antibody with Blotto. Cy3 donkey anti-mouse (Jackson Immunoresearch ) 1:500, FITC goat anti-mouse 1:500
) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
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