- ￥1338 - 11148
- abinScience
- France
- VK591013
- 2025年07月12日
- ELISA
- Alpaca
- Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebolavirus)
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
VHH-8His-Cys-tag
- 形态：
Liquid
- 保存条件：
-20°C
- 克隆性：
单克隆
- 适应物种：
Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebolavirus)
- 保质期：
12 months
- 级别：
一抗
- 供应商：
武汉益普生物科技有限公司
- 宿主：
Alpaca
- 应用范围：
ELISA
- 浓度：
1 mg/ml
- 抗体英文名：
Anti-SEBOV RNA-directed RNA polymerase L/Protein L Nanobody (SAA1489)
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1338.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥2288.0 |
| 规格： | 1mg | 产品价格： | ￥11148.0 |
| Product name | Anti-SEBOV RNA-directed RNA polymerase L/Protein L Nanobody (SAA1489) |
| Catalog No. | VK591013 |
| Target | L, RNA-directed RNA polymerase L, Protein L, Large structural protein, Replicase, Transcriptase |
| Clonality | Monoclonal |
| Host species | Alpaca |
| Species reactivity | Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebolavirus) |
| Applications | ELISA |
| Isotype | VHH-8His-Cys-tag |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Purified by Nickel column. |
| Endotoxin level | Please contact with the lab for this information. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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文献和实验in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
mRNA Amplification with T7 RNA Polymerase
RNase H Vortex master mix briefly, pull down by spinning briefly and place on ice. Add 80 µL 2nd Strand Master Mix to each RNA sample. Mix by pipetting, flicking and spin briefly to pull down. Incubate RNA at 16ºC x 2 hrs on PCR
一、文献 1. Chen, H.H., J. Lebon, and A.D. Riggs, Analysis of RNA structure and RNA-protein interactions in mammalian cells by use of terminal transferase-dependent PCR. Methods Mol Biol, 2008. 488: p. 319-41. 2. Terzi, L.C. and G.G. Simpson
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