- ￥1338 - 11148
- abinScience
- France
- HP069013
- 2025年07月10日
- ELISA, IF, IHC, WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
-20°C
- 克隆性：
单克隆
- 适应物种：
Human
- 保质期：
12 months
- 级别：
一抗
- 供应商：
武汉益普生物科技有限公司
- 宿主：
Mouse
- 应用范围：
ELISA, IF, IHC, WB
- 浓度：
1 mg/ml
- 抗体英文名：
Anti-Human CREPT Antibody (3E10#)
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1338.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥2288.0 |
| 规格： | 1mg | 产品价格： | ￥11148.0 |
| Product name | Anti-Human CREPT Antibody (3E10#) |
| Catalog No. | HP069013 |
| Target | RPRD1B, CREPT, Regulation of nuclear pre-mRNA domain-containing protein 1B, Cell cycle-related and expression-elevated protein in tumor |
| Clonality | Monoclonal |
| Host species | Mouse |
| Species reactivity | Human |
| Applications | ELISA, IF, IHC, WB |
| Isotype | IgG2a |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |
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文献和实验ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
in TE/0.1% BSA/0.1% azide) 2. Incubate 2 hr. @ RT on Nutator. Pam's note: If using monoclonal antibodies, make sure they are of an isotype recognized by Protein A-might need to use Protein G beads instead (e.g. for anti-myc 9E10). o Wash IP's Try
Wholemount in situ hybridisation
D. PREABSORPTION OF ANTIBODY 1 During the washing of the embryos (step 1 above), weigh out 3mg of embryo powder into a microtube, add 0.5mL of 10% sheep serum, 2% BSA in TBTX and 1µL anti-DIG-AP Fab fragment (Boehringer
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
, then sink in 30% sucrose in PBS + Mg2+at 4O C. Fixation times will vary with the size and nature of the tissue. For embryonic chick brains of E10 or older and for postnatal rat or mouse brains we incubate up to 8 hours in fixative. Perfusion
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