Anti-Vaccinia virus/VACV A27L

E L I S A w i t h p l a t e l e t s

for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS

Generation of Antibody Molecules Through Antibody Engineering

domains of the heavy (V H ) and light (V L ) chains. Effector functions are determined by constant C H 2 and C H 3 domains. The picture is based on the crystal structure of an intact IgG2 anti-canine lymphoma MAb231 ( 2 ) (pdb entry 1IGT). The drawing

Anti-GFP/BrdU In Situ Immunostaining

by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v,  plus 1% goat serum in PBS) and incubate 1 hr. at room temp.  Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp.  Wash 3x in PBS. Denature DNA with 1 mL