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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
单克隆
- 适应物种:
Human
- 保质期:
12 months
- 级别:
一抗
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Mouse
- 应用范围:
ELISA
- 浓度:
1 mg/ml
- 抗体英文名:
Anti-Human COL2A1 Antibody (GB8)
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1338.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2288.0 |
| 规格: | 1mg | 产品价格: | ¥11148.0 |

| Product name | Anti-Human COL2A1 Antibody (GB8) |
| Catalog No. | HF515053 |
| Target | COL2A1, Collagen alpha-1(II) chain, Alpha-1 type II collagen |
| Clonality | Monoclonal |
| Host species | Mouse |
| Species reactivity | Human |
| Applications | ELISA |
| Isotype | IgG1 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | For research use only. |

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文献和实验Intracellular Immunofluorescent Staining for Flow Cytometry
supernatant. [OPTIONAL] Block with 2% (2 µl) normal rat serum in 1X Permeabilization Buffer, in approximately 100 µl volume, at 4°C for 15 minutes. Without washing after blocking step, add 20 µl fluorochrome conjugated anti-human Foxp3 antibody
Southern Blotting: DNA Transfer
mM MgCl2 Antibody: 10 µl anti-digoxigenin-AP Fab fragments (Boehringer-Mann cat#1093274) 40ml of Buffer #1 10ml of Buffer #2 This will last several months of reuse if stored at 4℃.CSPD Ready-to-Use: Keep reused portions separate from used. Store
Southern Blotting(Fred Hutchinson Cancer Research Cente
#2: 2% Blocking Reagent in Buffer #1 Heat to 50-70℃; prepare ~60' in advance; sol'n remains turbid Store at 4℃. Add NaAzide for long term storage. Buffer #3: 100mM Tris-Cl, pH 9.5 100mM NaCl 50mM MgCl2 Antibody: 10 %26micro;l anti-digoxigenin-AP Fab
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