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文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
during the extended incubation period. Crosses are made on petri dishes for specific purposes, as when shot asci are to be collected. But plates are more prone to contamination and desiccation than tubes, and less compact for incubation and storage. 2. Media
Hints and precautions for the care, feeding and breeding of Neurospora
with cross tubes because they result in more rapid water loss and are more prone to contamination during the extended incubation period. Crosses are made on petri dishes for specific purposes, as when shot asci are to be collected. But plates are more prone
Methods for use with the mTn-3xHA/lacZ-mutagenized library
from these transformants and cut with a 'recovery' enzyme (Eco RI, Hin dIII, Cla I, Sal I, Xho I, Kpn I). This releases as a linear segment the bacterial origin of replication, the beta-lactamase gene and a portion of the lacZ gene with adjacent yeast DNA; this fragment
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