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文献和实验Determining the Direction of Replication Fork Movement
, with the enzyme of choice in various brands of agarose. The enzymes that cut to completion in Beckman LE agarose are AvaI, Bam HI, BglII, EcoRV, NcoI, PstI, PvuII, SacII, SnaBI, SpeI, StuI, XbaI and XhoI. (There are likely to be many others that also cut
ligate 3 DNA fragments with sticky ends into Vector-Mole
Hi, Dear All, Have you ever tried to ligate 3 DNA fragments with sticky ends into Vector(5.2kb). A. Fragment: 3.0kb, with HindIII/ Bam HI cut from a Vector. B. Fragment: 0.5kb, BamHI / XbaI cut from a Vector. C. Fragment: 3.5kb, XbaI/ NotI cut
of choice in various brands of agarose. The enzymes that cut to completion in Beckman LE agarose are AvaI, Bam HI, BglII, EcoRV, NcoI, PstI, PvuII, SacII, SnaBI, SpeI, StuI, XbaI and XhoI. (There are likely to be many others that also cut to completion
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