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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
from palindromic recognition sites are preferred in 3C assays, and the six-cutters such as EcoRI, HindIII, and BglII are commonly used. These should cut every 4 kb along the genomic DNA and offer a good starting point. To obtain higher-resolution maps, frequent
AFLP: not only for fingerprinting, but for positional cloning
, one EcoRI (having a 6 bp recognition site) and the other MseI (having a 4 bp recognition site). EcoRI cuts ~ every 2-2.5 Kb in Arabidopsis, while MseI cuts ~ every 300-400 bp. Thus a large number of fragments are generated with relative abundances
AFLP: not only for fingerprinting, but for positional cloning
from each ecotype is digested to completion with two restriction enzymes, one EcoRI (having a 6 bp recognition site) and the other MseI (having a 4 bp recognition site). EcoRI cuts ~ every 2-2.5 Kb in Arabidopsis, while MseI cuts ~ every 300-400 bp. Thus a large
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