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文献和实验ligate 3 DNA fragments with sticky ends into Vector-Mole
Hi, Dear All, Have you ever tried to ligate 3 DNA fragments with sticky ends into Vector(5.2kb). A. Fragment: 3.0kb, with HindIII/ Bam HI cut from a Vector. B. Fragment: 0.5kb, BamHI / XbaI cut from a Vector. C. Fragment: 3.5kb, XbaI/ NotI cut
Southern Blot Analysis(from Baker lab, university of Florida
2.Prepare 32P labeled 8/HindIII molecular weight standard. Desire 10-20Kcpm to be loaded with 1Fg of cold standard for Ethidium Bromidevisualization. 3.Load gel under fresh 1X TBE and run slowly (100V) to avoid smearing ofsamples
Southern Blot Analysis(from Baker lab, university of Florida)
trapped underneath comb and that the all bubbles on the surface ofthe agarose are removed before the gel sets.A16-well comb will allow theloading of approximately 30-35μl of sample.2.Prepare 32P labeled 8/HindIII molecular weight standard.Desire 10-20Kcpm
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