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文献和实验An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources
The Transdirect insect cell is a newly developed in vitro translation system for mRNA templates, which utilizes an extract from cultured Spodoptera frugiperda 21 (Sf21) insect cells. An expression vector, pTD1, which includes a 5′
Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates
Production and Functional Assays of Recombinant Secreted Amyloid Precursor Protein (APP) (sAPPα)
The β-amyloid precursor protein (APP) is connected to Alzheimer’s disease by both biochemistry and genetics. As the source of the major constituent of amyloid plaques, APP has been the subject of many studies of its expression and metabolism
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