Invent Minute胶片中蛋白质、核酸提取试剂盒 M
inute Protein/Nucleic Acid Extraction Kit from Gels 货号:PN-019- ￥1500 - 8000
- Invent
- 美国
- PN-019
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
室温
- 保质期：
见包装
- 英文名：
Invent Minute Protein/Nucleic Acid Extraction Kit from Gels
- 库存：
999
- 供应商：
北京泽平
- 规格：
20次
货号：PN-019
中文名称：Minute胶片中蛋白质、核酸提取试剂盒
英文名称：Minute Protein/Nucleic Acid Extraction Kit from Gels
包装规格：20次
品牌：英文特 Invent Biotechnologies
产地：美国
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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
temperature for 5 minutes. 9. Centrifuge at maximum speed ($13,000 x g) at 4°C for 10 minutes to precipitate Nucleic acid. Discord the supernatant. 10. Wash the nucleic acid pellet by adding 400 μL of 70% ethanol into the tube. Vortex the tube
PMSF (1 mM) Protease Inhibitor Cocktail, Sigma (Cat. no. P-2714) Quant-iT™ Protein Quantitation Kit (Cat. no. Q33210) Or Cell Extraction Buffer Formulation 100 mM Tris, pH 7.4 2 mM Na3 VO4 100 mM NaCl
Genomic DNA Extraction - PureLink
. Add 200 µl Binding Buffer (L3) supplied with the kit to the lysate. Mix well by vortexing for 5 seconds. The SDS precipitates in the presence of guanidine isothiocyanate. Incubate at 70°C for 10 minutes to solubilize SDS and promote protein
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