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- Nepa Electroporation Cuvettes are designed to maximize molecular electroporation efficiencies for bacteria, yeast, mammalian and plant cells. Each batch of cuvettes has to undergo rigorous testing at several stages during the manufacturing process for engineering tolerances, biocompatibility and sterility, prior their being quality tested for optimal and reproducible impedance measurements.
- All batches are checked to optimize the bio and transfection compatibility, with stringent use of high quality grade polycarbonate and High grade chemicals to ensure consistent uniform pulse generation and improved gene transfer.
- All batches are checked to optimize the bio and transfection compatibility, with stringent use of high quality grade polycarbonate and High grade chemicals to ensure consistent uniform pulse generation and improved gene transfer.
- Every cuvette is guaranteed sterile, packed using gamma irradiation and has a simple tear wrapper for easy access when you need it.
- Nepa Electroporation Cuvettes are compatible with most electroporation systems (Bio-Rad, BTX, etc.).
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文献和实验Mesoplasma florum:Electroporation
level. Select cultures which are just changing color. chilled mycoplasma electroporation buffer 8 mM HEPES pH 7.4 272 mM sucrose chilled 1 mm electroporation cuvettes 10 ml chilled ATCC 1161 medium
Streptomyces:Protocols/Transformation by Electroporation
spreader. Dry the plates so the liquid is not visible. Incubate @ 37°C overnight. Notes Set the BioRad MicroPulser to the correct setting for the cuvettes being used, i.e. EC1 for BioRad brown capped (1mm) cuvettes or EC2 for green
Transformation of E. coli by Electroporation
, together with an appropriate number of bacterial electroporation cuvettes. 13. Add 10 pg to 25 ng of the DNA to be electroporated in a volume of 1-2 μl to each microfuge tube and incubate the tube on ice for 30-60 seconds. Include all of the appropriate positive
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