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文献和实验Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
Affinity Purification of RNA Using an ARiBo Tag
tag contains the λboxB RNA and the glmS ribozyme, allowing immobilization on GSH-Sepharose resin via a λN-GST fusion protein and elution by activation of the glmS ribozyme with glucosamine-6-phosphate. This Chapter outlines the experimental details
【Jounal club】常用标签Tag的比较: 纯度,收率,载量,成本等
of eight elutable Affinity tags to purify proteins from Escherichia coli, yeast, Drosophila, and HeLa extracts. Our results show that the HIS, CBP, CYD (covalent yet dissociable NorpD peptide), Strep II, FLAG, HPC (heavy chain of protein C) peptide tags, and the GST
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