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Hepatocyte growth factor (HGF) is a potent epithelial mitogen whose actions are mediated through its receptor, the proto-oncogene c-Met. Full-length HGF is a 90-kD protein composed of an NH2-terminal domain, four consecutive kringle domains, and a carboxy-terminal protease-like domain. NK1 was converted to an agonist by adding heparin to the culture medium. The interaction of NK1 and heparin was further studied in BaF3 cells, which express little or no cell surface heparan sulfate proteoglycans. In BaF3 cells transfected with a plasmid encoding human c-Met, heparin and NK1 synergized to stimulate DNA synthesis and cell proliferation.
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文献和实验Rapid Separation of Protein‐Bound DNA from Free DNA Using Nitrocellulose Filters
‐stranded, 32 P‐labeled DNA Protein sample Bovine serum albumin (BSA; optional
Purification of Endotoxin Free mAbs
1) Endotoxin - you must assume that all standard laboratory glassware will be coated with loads of endotoxin. Wherever possible use sterile, specifically endotoxin-free (clinical grade) disposable plastics. If you need to use glassware or other materials
Lowry Protein AssayLaboratory of P.J.Hansen,Dept.of Animal Sciences,University of FloridaThe Lowry procedure is one of the most venerable and widely-used protein assays,being first described in 1951 [Lowry et al.,J.Biol.Chem.193: 265-275 (1951
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