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Cytokines are considered important factors in the modulation of various immune responses. Among them, interleukin (IL)-21 is one of the major immune modulators, adjusting various immune responses by affecting various immune cells. It has been suggested that IL-21 may enhance autoimmunity through different mechanisms, such as development and activation of helper T (TH)-17 and follicular helper T (TFH) cells, activation of natural killer (NK) cells, enhancing B-cell differentiation and antibody secretion and suppression of regulatory T (Treg) cells. Actually IL-21 is a pleiotropic cytokine whose functions in protection and immunopathology during parasitic diseases have been explored in limited ways, and it has been revealed to elicit antitumor-immune responses in several tumor models.
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文献和实验intracellular nucleases and the subsequent isolation of intact nucleic acids. Following digestion, degraded protein is routinely removed by phenol extraction. For example, proteinase K has been used to degrade protein during the isolation of high-mol-wt
6. Quant-iT™ Protein Quantitation Kit (Cat. no. Q33210) 实验设备 5 mL Culture Tubes 实验步骤 1. Remove liver aseptically. 2. Flash-freeze liver in foil packets using liquid nitrogen. Store
Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions
Optimizing the conditions for the overexpression of membrane proteins in E . coli and their subsequent purification is usually a laborious and time-consuming process. Combining the Lemo21(DE3) strain, which conveniently allows to identify
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