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CD49f, also known as integrin α6 subunit, is a type I transmembrane glycoprotein encoded by the ITGA6 gene. It belongs to the α-subfamily of integrins and can form heterodimers with β1 (CD29) or β4 (CD104) subunits to create functional receptors such as α6β1 or α6β4. These receptors play crucial roles in cell adhesion, migration, and signaling. CD49f is highly expressed in various cell types, including T cells, monocytes, platelets, epithelial cells, and certain stem cells. In cancer biology, CD49f has been identified as a marker for tumor-initiating cells (TICs) in hepatocellular carcinoma (HCC), where it is associated with cell stemness, immune evasion, and the formation of an immunosuppressive tumor microenvironment. Additionally, CD49f has been used as a marker for human astrocytes and has shown potential in studying neurodegenerative diseases.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
的优点就是对光稳定性，Cy5 和 AlexaFluor 647 以花青素为基础，因此有着和花青素一样的特点即耐光性较差。极好的亮度和耐光性使CF ? 640R被理想的用于共聚焦显微镜和单分子成像等条件苛刻的检测中。CF 647、AlexaFluor 647, ATTO 647N, DyLight649等远红外荧光染料；以花青素为基础的远红外荧光染料CF647 比Cy 5、Alexa Fluor 647 明亮。CF 660、AlexaFluor® 660, Allophycocyanin (APC
® 555 Conjugate）#4409 Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 647 Conjugate）#4410 抗大鼠： Anti-Rat IgG（H+L），（Alexa Fluor® 488 Conjugate）#4416 Anti-Rat IgG（H+L），（Alexa Fluor® 555 Conjugate）#4417 Anti-Rat IgG（H+L），（Alexa
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