
通用型免疫(共)沉淀(IP/Co-IP)工具箱(磁珠)
研选同类产品更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 保存条件:
依据产品说明建议保存条件储存。
- 保质期:
依据产品说明建议保存条件储存。
- 英文名:
Universal IP/Co-IP Toolkit (Magnetic Beads)
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
20 T
通用型免疫(共)沉淀(IP/Co-IP)工具箱(磁珠)
货号:KTD104 | 规格:20 T | 价格:¥998 | 货期:现货(当天发货)
◆ 核心定位
蛋白研究黄金搭档 | 专为IP/Co-IP/WB全流程设计的磁珠体系工具箱,适配小鼠/兔源抗体,满足样品制备-抗原富集-检测分析一站式需求。
◆ 试剂盒组分
- Non-Denaturing Lysis Buffer-25 mL
- Denaturing Lysis Buffer-25 mL
- 10×Wash Buffer-20 mL
- Protein A/G Magnetic Beads-0.45 mL
- Elution Buffer-2 mL
- Neutralization Buffer-0.2 mL
- 100×Proteinase Inhibitor Cocktail-0.2 mL
- Mouse IgG (1 mg/mL)-30 μL
- Rabbit IgG (1 mg/mL)-30 μL
- IPKine™ HRP, Goat Anti-Mouse IgG LCS-30 μL
- IPKine™ HRP, Mouse Anti-Rabbit IgG LCS-30 μL
◆ 技术优势
✓ 抗体经济性:磁珠抗体结合效率提升30%,抗体用量节省50%
✓ 双模式兼容:非变性/变性裂解双缓冲液体系,适配天然/变性蛋白样本
✓ 零背景干扰:内源性IgG阻断技术+IPKine™ HRP二抗,彻底消除重链干扰
✓ 全流程质控:标配阴性对照IgG,支持抗体特异性验证及假阳性排查
◆ 应用场景
IP/Co-IP:蛋白质互作验证 | 复合体富集
WB前处理:低丰度靶标浓缩 | 复杂样本纯化
▍保存与运输
保存条件:2-8℃避光保存(详见产品说明)
运输方案:蓝冰冷链运输(全程4-10℃温控)
▍质量承诺
磁珠粒径均一性:CV<5% | 批次间一致性:>98% | 蛋白回收率:>85%
⚠ 警示声明:
本产品仅供科研使用,不可用于临床诊断。使用前请查阅所有相关专利及许可协议,因违规使用导致的任何法律责任均由用户自行承担。
```
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Universal Immunoprecipitation (IP/Co-IP) Toolbox (Magnetic Beads)
Catalog No.: KTD104 | Specification: 20 T | Price: ¥998 | Availability: In stock (shipped same day)
◆ Core Positioning
Gold-standard partner for protein research | A magnetic bead system toolbox specifically designed for IP/Co-IP/WB workflows, compatible with mouse/rabbit-derived antibodies, meeting one-stop needs for sample preparation-antigen enrichment-detection analysis.
◆ Kit Components
- Non-Denaturing Lysis Buffer-25 mL
- Denaturing Lysis Buffer-25 mL
- 10×Wash Buffer-20 mL
- Protein A/G Magnetic Beads-0.45 mL
- Elution Buffer-2 mL
- Neutralization Buffer-0.2 mL
- 100×Proteinase Inhibitor Cocktail-0.2 mL
- Mouse IgG (1 mg/mL)-30 μL
- Rabbit IgG (1 mg/mL)-30 μL
- IPKine™ HRP, Goat Anti-Mouse IgG LCS-30 μL
- IPKine™ HRP, Mouse Anti-Rabbit IgG LCS-30 μL
◆ Technical Advantages
✓ Antibody economy: 30% increased antibody-binding efficiency with magnetic beads, 50% antibody consumption reduction
✓ Dual-mode compatibility: Non-denaturing/denaturing dual lysis buffer systems, suitable for native/denatured protein samples
✓ Zero background interference: Endogenous IgG blocking technology + IPKine™ HRP secondary antibodies, completely eliminating heavy chain interference
✓ Full-process QC: Standard negative control IgG included, supporting antibody specificity validation and false-positive screening
◆ Applications
IP/Co-IP: Protein-protein interaction validation | Complex enrichment
WB pre-treatment: Low-abundance target concentration | Complex sample purification
▍Storage & Shipping
Storage conditions: 2-8°C protected from light (see manual for details)
Shipping protocol: Blue ice cold chain transport (4-10°C temperature control throughout)
▍Quality Commitment
Magnetic bead size uniformity: CV<5% | Batch-to-batch consistency: >98% | Protein recovery rate: >85%
⚠ Warning Statement:
This product is for research use only, not for clinical diagnosis. Please review all relevant patents and license agreements before use. Users assume full legal responsibility for any liabilities arising from non-compliant usage.
```
Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult Yakonin Company.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
用户评价
暂无用户评价
文献和实验Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop.
作者:Yang, Sheng, et al. 杂志名称:Experimental & Molecular Medicine IF:5
Geniposide ameliorates atherosclerosis by restoring lipophagy via suppressing PARP1/PI3K/AKT signaling pathway.
作者:Lin, Jinhai, et al. 杂志名称: Phytomedicine IF:7
Huanglian Jiedu decoction inhibits vascular smooth muscle cell-derived foam cell formation by activating autophagy via suppressing P2RY12.
作者:Lin, Jinhai, et al. 杂志名称:Journal of Ethnopharmacology IF:5
了一线技术支持常被问到的问题,希望这篇纯干货能帮助大家顺利升级打怪,早日驾驭 IP、co-IP。 IP、co-IP、pull-down 简介 IP(Immunoprecipitation),免疫沉淀:是利用固定在磁珠或琼脂糖树脂等基质上的特异性抗体对抗原进行小型亲和纯化的一种方法 Co-IP(co-Immunoprecipitation),免疫共沉淀: co-IP 是一种常用的鉴定蛋白-蛋白相互作用的方法,其基本实验流程和IP类似,通过使用诱饵蛋白(IP 中的抗原,bait protein)特异
蛋白质-蛋白质相互作用(PPI)对解析生命活动过程与疾病发生发展过程至关重要。随着时代发展,虽然大规模、高通量的生物学研究手段大大促进了蛋白质相互作用的预测,但这种预测还需要进一步利用体外和体内系统进行验证。分析蛋白质互作的方法多种多样,本篇文章为大家介绍以下几种互作分析技术: 免疫共沉淀 Co-IP 免疫沉淀 (IP)、免疫共沉淀 (Co-IP)和 pull-down 都是常用的从复杂的混合样品中获取目标分子,以进行下一步分析的方法,如检测蛋白质能否相互作用。在此类实验中,被研究的蛋白质称为
了编码 HA-BDCA-2 载体的小鼠 B 前体细胞后,提取的蛋白使用 SDS-PAGE 的方法进行检测。 蛋白免疫共沉淀(co-IP)与染色质免疫共沉淀(ChIP) μMACS Protein A/G MicroBeads 专为蛋白质免疫共沉淀分析而开发,免疫复合物 30 分钟即可形成并得到磁性分离。 在强磁场的分离柱中进行润洗,可保证背景充分去除,即便是弱结合的蛋白复合物依然可得到充分分离。传统免疫共沉淀工作流程需要抗体孵育过夜,相比之下 MACS Techology 可令实验时间缩短
技术资料暂无技术资料 索取技术资料









