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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 英文名:
Mouse Flt-3 Ligand protein
- 规格:
5 μg/20 μg/100 μg/1 mg
| 规格: | 5 μg | 产品价格: | ¥588.0 |
|---|---|---|---|
| 规格: | 20 μg | 产品价格: | ¥1498.0 |
| 规格: | 100 μg | 产品价格: | ¥3998.0 |
| 规格: | 1 mg | 产品价格: | 询价 |
重组小鼠Fms相关酪氨酸激酶3配体(Flt-3 Ligand)
货号:PRP1149 | 产品类型:细胞因子
▍产品概述
本产品为大肠杆菌表达的重组小鼠源Flt-3配体蛋白片段(Gly27-Arg188),C端携带6×His标签,经SDS-PAGE验证纯度>98%,分子量19.3 kDa。专为造血祖细胞增殖研究设计,ED50<2 ng/mL的生物活性确保实验可靠性。
▍核心参数
- 活性验证:诱导转染小鼠Flt-3的BaF3细胞增殖,ED50<2 ng/mL
- 超低内毒素:<0.1 EU/μg(LAL法检测)
- 种属特异性:小鼠源(UniProt ID:P49772)
- 稳定性:-20°C保存1年,复溶后4°C稳定1周
▍技术规格
| 表达系统 | 大肠杆菌 |
|---|---|
| 蛋白长度 | 19.3 kDa(还原条件SDS-PAGE 19 kDa) |
| 缓冲体系 | 冻干粉含1×PBS(pH7.4) |
| 复溶建议 | 无菌10 mM HCl配制≥100 μg/mL,静置20分钟 |
▍订购信息
- 5 μg | ¥588 | 现货
- 20 μg | ¥1498 | 现货
- 100 μg | ¥3998 | 3-5工作日
- 定制规格 | 专属方案 | 技术咨询
⚠ 重要操作提示
1. 开盖前3000 rpm离心5分钟
2. 复溶过程禁止振荡
3. 长期保存需添加载体蛋白(0.1% BSA/5%海藻糖)
4. 应用范围:科研级(不适用于临床诊断)
※ 本产品诱导的细胞增殖效应需与GM-CSF、IL-3等因子协同验证,具体实验方案请联系技术支持获取。
```注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Recombinant Mouse Fms-Related Tyrosine Kinase 3 Ligand (Flt-3 Ligand)
Catalog Number: PRP1149 | Product Type: Cytokine
▍Product Overview
This product is a recombinant mouse-derived Flt-3 ligand protein fragment (Gly27-Arg188) expressed in E. coli, with a C-terminal 6×His tag. The purity is verified by SDS-PAGE to be >98%, with a molecular weight of 19.3 kDa. It is specifically designed for hematopoietic progenitor cell proliferation studies, with a biological activity of ED50<2 ng/mL ensuring experimental reliability.
▍Core Parameters
- Activity Validation: Induces proliferation of BaF3 cells transfected with mouse Flt-3, ED50<2 ng/mL
- Ultra-Low Endotoxin:<0.1 EU/μg (detected by LAL method)
- Species Specificity: Mouse-derived (UniProt ID: P49772)
- Stability: Stable for 1 year at -20°C, and 1 week at 4°C after reconstitution
▍Technical Specifications
| Expression System | E. coli |
|---|---|
| Protein Length | 19.3 kDa (19 kDa under reducing conditions by SDS-PAGE) |
| Buffer System | Lyophilized powder containing 1×PBS (pH7.4) |
| Reconstitution Recommendation | Reconstitute in sterile 10 mM HCl to ≥100 μg/mL, let stand for 20 minutes |
▍Ordering Information
- 5 μg | ¥588 | In Stock
- 20 μg | ¥1498 | In Stock
- 100 μg | ¥3998 | 3-5 Working Days
- Custom Specifications | Custom Solutions | Technical Consultation
⚠ Important Operation Tips
1. Centrifuge at 3000 rpm for 5 minutes before opening
2. Do not vortex during reconstitution
3. Add carrier protein (0.1% BSA/5% trehalose) for long-term storage
4. Application Scope: Research Grade (Not for clinical diagnosis)
※ The cell proliferation effect induced by this product needs to be verified in synergy with factors such as GM-CSF and IL-3. Please contact technical support for specific experimental protocols.
Note: The above information may change due to product optimization and upgrades. For specific information, please consult Abbkine Company.
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