EliKine™ 人肝细胞生长因子 ELISA定量试剂盒

EliKine™ 人肝细胞生长因子（HGF）ELISA定量试剂盒

 产品概述

KTE6009采用双抗体夹心法原理，精准定量检测人源样本中125-8000 pg/mL范围的HGF蛋白，灵敏度达60 pg/mL，满足细胞培养上清、血清、血浆等多生物液体分析需求。

⚙️ 技术参数

• ▶ 检测范围：125-8000 pg/mL
• ▶ 最低检出限：60 pg/mL
• ▶ 板内变异系数：<8%｜板间变异系数：<10%

 产品优势

零交叉反应设计通过ISO 13485认证，预包被微孔板采用真空铝箔封装技术，配套EliKine™ Streptavidin-HRP增强系统，批间差控制达国际领先水平。

離 兼容样本

• • 细胞培养上清液
• • 血清/血浆（推荐1:20稀释）
• • 组织裂解液（需离心预处理）

 试剂盒组分

⚠️ 实验质控要点

• • 推荐三复孔检测模式
• • 振荡孵育参数：500rpm/25℃
• • 显色终止15分钟内完成读数

❄️ 保存方案

• 未开封：2-8℃直立保存（有效期18个月）
• 已开封：微孔板干燥剂密封，检测抗体-20℃分装

 背景解析

HGF（P14210）通过c-Met受体介导上皮-间质转化，在肿瘤微环境调控和组织再生中起核心作用，其异常表达与肝癌、肺纤维化等疾病密切相关（Gene ID:3082）。

 订购信息

规格	48T	96T
价格	￥1598	￥2558
货期	现货（冷链运输次日达）

*本产品仅用于科研，不适用于临床诊断。专利状态请咨询当地监管机构。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Human Hepatocyte Growth Factor (HGF) ELISA Quantitation Kit

 Product Overview

KTE6009 employs a sandwich ELISA principle to accurately quantify HGF protein in human samples within the range of 125-8000 pg/mL, with a sensitivity of 60 pg/mL. It is suitable for analyzing various biological fluids including cell culture supernatants, serum, and plasma.

⚙️ Technical Parameters

• ▶ Detection Range: 125-8000 pg/mL
• ▶ Minimum Detectable Limit: 60 pg/mL
• ▶ Intra-assay CV: <8%｜Inter-assay CV: <10%

 Product Advantages

Zero cross-reactivity design certified by ISO 13485. Pre-coated microplates utilize vacuum aluminum foil packaging technology, paired with the EliKine™ Streptavidin-HRP enhancement system, achieving internationally leading inter-batch variability control.

離 Compatible Samples

• • Cell culture supernatant
• • Serum/Plasma (recommended 1:20 dilution)
• • Tissue lysate (requires pre-centrifugation)

 Kit Components

⚠️ Key QC Points

• • Recommended triplicate detection mode
• • Incubation parameters: 500rpm/25℃
• • Complete reading within 15 minutes after stopping the reaction

❄️ Storage Conditions

• Unopened: Store upright at 2-8℃ (18-month shelf life)
• Opened: Microplates should be sealed with desiccant, detection antibody should be aliquoted and stored at -20℃

 Background

HGF (P14210) mediates epithelial-mesenchymal transition through c-Met receptor, playing a central role in tumor microenvironment regulation and tissue regeneration. Its abnormal expression is closely associated with diseases such as liver cancer and pulmonary fibrosis (Gene ID:3082).

 Ordering Information

Specification	48T	96T
Price	¥1598	¥2558
Lead Time	In stock (cold chain delivery next-day available)

*For research use only. Not for clinical diagnosis. Patent status should be verified with local regulatory agencies.

Note: The above information may change due to product optimization and upgrades. Please consult Abbkine for specific details.