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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
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- 英文名：
Latex Microsphere *120 nm, White, COOH, 10%*
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
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- 规格：
10mL
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文献和实验【共享】Present and future applications of gold in rapid assays
. Visibility. Gold particles in the range of 20–120 nm appear bright red because they efficiently scatter green light. Even though the particles are smaller than the wavelength of incident light, the scatter occurs due to the property of surface plasmon
briefly. Then add 25 ul of 10% SDS and 5 ul of proteinase K (20 mg/ml H2O) (Sigma P-0390), vortex briefly and incubate for 1 hour at 55degC. 6. Add 120 ul phenol/chloroform/isoamyl alcohol and vortex for 30 seconds. Centrifuge the sample for 2 minutes
一、微量凯氏（kjeldahl）定氮法样品与浓硫酸共热。含氮有机物即分解产生氨（消化），氨又与硫酸作用，变成硫酸氨。经强碱碱化使之分解放出氨，借蒸汽将氨蒸至酸液中，根据此酸液被中和的程度可计算得样品之氮含量。若以甘氨酸为例，其反应式如下：NH2 CH2 COOH+3H2 SO4 ――2CO2 +3SO2 +4H2O+NH3 (1)2NH3 +H2 SO4 ――(NH4 )2 SO4 (2)(NH4 )2 SO4 +2NaOH――2H2 O+Na2 SO4 +2NH3 (3)反应
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