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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
【干冰】iFluor® 532-dUTP *1 mM in TE Buffer (pH 7.5)*
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
25nmoles
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文献和实验Preparation of Microinjection Buffer
Buffer composition is 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 30 microM spermine, 70 microM spermidine, 100 mM NaCl. This buffer is more likely to produce transgenic mice with intact, unfragmented DNA molecules. See the following references: Schedl
: 6.61 g Tris-HCl + 0.8 g Tris-Base (pH 7.4 at 25℃)C: 1 M MgCl2 10 ml: 2.03 gD: 3 M NaCl 20 ml: 3.50 gE: 2M KCl 10 ml: 1.48 gBan I: 0.3ml B + 0.3ml C + 0.10ml D + 0.1ml BSA + 21µl bME + 4.18ml H2OBgl II: 0.5ml B + 0.5ml C + 1.67ml D + 0.1ml BSA + 35µl bME
CCMB buffer to make chemical competent cell
does not appear to be necessary Test competence (see below) Good cells should yield around 100 - 400 colonies 4. CCMB80 buffer 10 mM KOAc pH 7.0 (10 ml of a 1M stock/l) 80 mM CaCl2.2H2O (11.8 g/l) 20 mM MnCl2.4H2O (4.0 g/l) 10 mM MgCl2.6H2O (2.0 g/l) 10
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