Amplite® Colorimetric Glyceral

Simultaneous Detection of Bluetongue Virus RNA, Internal Control GAPDH mRNA, and External Control Synthetic RNA by Multiplex Real-Time PCR

), whereas the internal and external RT-qPCRs were specifically designed to detect endogenous glyceraldehyde-3-phosphate dehydrogenase mRNA and a synthetic RNA, respectively. To maximize the sensitivity of the assay, the primer concentrations of the internal/external

Quantitation of Multiple RNA Species

-3-phosphate dehydrogenase (GAPDH) (3 ), and commercial standard kits for these are available. Different internal controls may be appropriate depending on the cell types or conditions being studied, and it is advisable to try several different controls

The Use of Comparative Quantitative RT-PCR to Investigate the Effect of Cysteine Incubation on GPx1 Expression in Freshly Isolated Cardiomyocytes

method is the conventional RT-PCR in a standard thermal cycler followed by electrophoresis and scanning densitometry using the expression of the housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), for normalising purposes. Each step