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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
iFluor® 500 succinimidyl ester
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
1mg
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
.Resuspend pellet in ~500-800 microliters of ice cold injection uffer.Incubate at 0℃ for 10 min. 19.Centrifuge in a SS34 rotor at 18,000 rpm,4℃ for 20 min 20.Drop freeze the labeled tubulin. 21.Final tubulin concentration is determined by the Lowry assay
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,1 mM PIPES,pH 6.8 at 4℃,250 ml for day 0. 2.0.5 mM ATP,0.2 mM CaCl2,70 mM KCl,150 mM PIPES,pH 7.0,1 ml. 3.16 mM ATP,0.2 mM CaCl2,5 mM DTT,5 mM lysine,10 mM glutamic acid,20 mM Tris-HCl,pH 8.0,10 ml
cross-linker, 1-pyrenebutanoic acid, succinimidyl ester. The technique effectively tethers the enzyme to the carbon nanotube which enhances bioelectrocatalysis, preserves the conductive nature of the carbon surface, and facilities direct electron
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