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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Alkyne Modifier II, NHS ester [PEG4 alkyne NHS ester]
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
25mg
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文献和实验alkyne?azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini?DNA duplexes. The reaction is carried out on 5??alkyne?3??azide?labeled hairpin loop oligonucleotides and proceeds in high yield under mild conditions in as little as 5
Revised amino-allyl labeling protocol
, all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cy-dyes from coupling to free amine groups in solution. 3. Add 450 m L water to each reaction. C. Cleanup Add 500 m L neutralized, diluted reaction
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
by addition of 15 mL 0.1 N HCl. To continue with the amino-allyl dye coupling procedure, all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cy-dyes from coupling to free amine groups in solution. 3. Add 450
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