XFD488 Hydroxylamine *Same Str

Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities

to the amber stop codon TAG. Proteins were purified with high yield and subsequently labeled with the hydroxylamine derivative of Alexa Fluor� 488 functioning as a fluorescent donor dye. The proteins were then labeled via maleimide coupling chemistry

secondary antibody review -- data from 99 publications

ref host class subclass conjugation experiment dilution detail supplier 1 goat     Alexa Fluor 488 immunocytochemistry 1:250 localization of tagged EVA-1 in 293T cell Molecular Probes 1 goat     Alexa Fluor 546 immunocytochemistry 1:250

Atomic Force Microscopy for Analyzing Metaphase Chromosomes: Comparison of AFM Images with Fluorescence Labeling Images of Banding Patterns

with 5-ethynyl-2′-deoxyuridine (EdU) during the late S phase. EdU-labeled DNA is detected in metaphase chromosomes using Alexa Fluor 488� azide, through the 1,3-dipolar cycloaddition reaction of organic azides with the terminal acetylene group of EdU. Chromosomes