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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
MM 2-10 [N-(3-Triethylammoniumpropyl)-4-(4-(diethylamino)styryl)pyridinium dibromide]
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
1mg
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文献和实验Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR
HCl. 5. Vortex. The DNA is now in a 20 mM Tris/0.1 mM EDTA at pH 8.1 with about 12.5 mM Na++ ions. That's pretty close to the 10 mM Tris/ 0.1 mM EDTA / pH 8 solution used to store DNA. 6. Use 1 µl for PCR 7. (May need to microfuge for 6 minutes
。 注:本培养基亦称Rappaport 10(R10)增菌液。
TOP10 chemically competent cells
10 mM KOAc pH 7.0 (10 ml of a 1M stock/L) 80 mM CaCl2 .2H2 O (11.8 g/L) 20 mM MnCl2 .4H2 O (4.0 g/L) 10 mM MgCl2 .6H2 O (2.0 g/L) 10% glycerol (100 ml/L) adjust pH DOWN to 6.4 with 0.1N HCl if necessary
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