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- 文献和实验
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- 保存条件：
详询
- 保质期：
详询
- 英文名：
FAM-cAMP PDE IV substrate *Green Fluorescence*
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
0.5umol
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文献和实验Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer
subunits of the main cAMP effector, the protein kinase A (PKA), fused with two suitable green fluorescent protein (GFP) mutants is used for measuring changes in fluorescence resonance energy transfer (FRET) that correlate with changes in intracellular cAMP
activities of N -acetylglucosaminyltransferases (GnTs). Here, we describe methods for large scale preparation of the oligosaccharide acceptor substrate, fluorescence-labeling of oligosaccharides by pyridylamination, quality control, and reversed phase HPLC
The SIGEX (substrate-induced gene expression) method is a novel approach for the screening of gene (genome) libraries. In addition to the commonly used function- and sequence-driven approaches to screening, SIGEX provides a third option
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