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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Trypan Blue, sodium salt *CAS 72-57-1*
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
100g
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文献和实验and resuspended in protein-free medium or salt solution prior to counting.Supplies & Equipment:Eppendorf tubes (1.5 ml)Micropipet (10µl)HemocytometerReagents:HBSS (Hanks' Balanced Salt Solution)sterile Trypan blue solution 0.4% (Sigma T-8154)Procedure:Prepare
(Hanks' Balanced Salt Solution) sterile Trypan blue solution 0.4% (Sigma T-8154) Procedure: Prepare a cell suspension in HBSS Transfer into Eppendorf tube: 0.5 ml of 0.4% Trypan blue solution 0.3 ml
In Vitro Transcription Assay (Run-off Assay) using Permeabil
permeabilization by taking 10 μl of the cell suspension and mixing it with Trypan Blue. Determine the percentage of cell viability by recording the percent of cells that exclude the blue dye. 8. Keep the cell suspension on ice. 9. Add one-one hundredth
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