Recombinant Human IL-3 Protein

纯度

> 95 % as determined by SDS-PAGE.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 1-5 ng/mL.
2.Loaded Recombinant Human IL-3R alpha/CD123 Protein, hFc Tag (Cat. No. 10518-H02H) on proA Biosensor, can bind Recombinant Human IL-3 Protein, His Tag (Cat. No. 11858-H08H) with an affinity constant of 0.497 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).

蛋白构建

A DNA sequence encoding the human IL3 (NP_000579.2) (Met1-Phe152) was expressed with a polyhistidine tag at the C-terminus.

Accession#

NP_000579.2

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Ala 20

分子量

The recombinant human IL3 consists 144 amino acids and predicts a molecular mass of 16.5 kDa.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.