Recombinant Rat DNAM-1/CD226 P

纯度

> 95 % as determined by SDS-PAGE.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

1.Measured by its binding ability in a functional ELISA.
2.Immobilized rat CD226-His(Cat:80369-R08H) at 10μg/mL (100μL/well) can bind mouse PVR-Fch(Cat:50259-M03H), the EC₅₀ of mouse PVR-Fch is 0.06-0.2 μg/mL.

蛋白构建

A DNA sequence encoding the rat Cd226 (NP_001100840.1) (Met1-Ile265) was expressed with a polyhistidine tag at the C-terminus.

Accession#

NP_001100840.1

表达宿主

HEK293 Cells

种属

Rat

预测 N 端

Glu 32

分子量

The recombinant rat Cd226 consists 245 amino acids and predicts a molecular mass of 28 kDa.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.