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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
室温,12个月
- 库存:
现货
- 供应商:
鹿森生物
- 规格:
500ml
用途:用于乙醇沉淀RNA等,乙酸钠又称NaAc即醋酸钠
注意事项:无菌溶液。主要由乙酸钠、冰乙酸组成,DEPC处理后,高压灭菌。
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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions Sohyun L. McElroy1 and Renee A. Reijo Pera Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine
Cell-free System for the examination of apoptotic activity in cytoplasmic extracts
of Lysis Buffer (containing 0.5 µg/ml proteinase K, freshly added) to the cell-free reaction. Incubate over night at 37°C. Add 40 µl of 3M NaOAc, pH8.0, mix. Add 900 µl of ice-cold 100% ethanol, mix
Isolation of cell nuclei for the application in the cell-free system
(0.88 M) in NB: About 500 µl lysate over 2 ml 30% sucrose in a 6 ml round bottomed PP centrifuge tube. Spin nuclei down at 800 g for 10 min; aspirate supernatant, then suck away the top layer and the interphase, thoroughly. Optional for higher
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